T7 SSB
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase, Phosphatase (-)
T4 DNA Polymerase
MMLV reverse transcriptase
Vaccinia Topoisomerase I
Protein Expression Testing
AKTA FPLC (ion-exchange, IMAC)
Protein Purification Process Development
Non-Tagged Protein Purification
Complete Batch Records
Manufacturing Pilot Process
Scale-Up
Custom Protein Formulations
Tissue Culture (primary cells, adherent and suspension cell lines)
Viability Assays (fluorescent, colormetric)
Custom Competent Cell Preparation
(chemical, electrocompetent, 96 well
format)
(chemical, electrocompetent, 96 well
format)
Subcloning
Bacterial or Yeast Growth
Custom Protein Formulations
Ф29 (Phi-29) DNA Polymerase
| Cat. number | Concentration (U/ul) | Size (ul) | Size (units) | Price (US Dollar) | |
| 4001 | 10 | 100 | 1,000 | $120.00 |  |
| 4002 | 100 | 100 | 10,000 | $700.00 | |
| 4003 | 10 | 1,000 | 10,000 | $700.00 | |
You can also place an order by faxing a completed order form to 858-558-3740 or calling us at 858-558-3702.
Description:
This Ф29 DNA polymerase is derived from the replicative polymerase of the B. subtillis bacteriophage Ф29 and expressed in E. coli. This polymerase exhibits high processivity and strong strand-displacement properties as well as an inherent 3' to 5' proofreading exonuclease activity.
Available in two concentrations and supplied with 10X reaction buffer.
The higher-concentration (100 U/µl) enzyme is also supplied with 1X dilution buffer.
Protein Uses:
Rolling circle amplification (RCA).
Multiple displacement amplification (MDA).
Whole genome amplification.
Protein-primed DNA amplification.
In situ genotyping using padlock probes.
Additional Information:
Single recombinant polypeptide of 66 kDa, expressed in E.coli.
High purity, low DNA content.
Capable of synthesizing DNA fragments of > 70 kb.
Specific activity ≥ 100,000 U/mg.
Capable of incorporating 2,000 nucleotides/minute @ 34°C.