T7 SSB
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase, Phosphatase (-)
T4 DNA Polymerase
MMLV reverse transcriptase
Vaccinia Topoisomerase I
Protein Expression Testing
AKTA FPLC (ion-exchange, IMAC)
Protein Purification Process Development
Non-Tagged Protein Purification
Complete Batch Records
Manufacturing Pilot Process
Scale-Up
Custom Protein Formulations
Tissue Culture (primary cells, adherent and suspension cell lines)
Viability Assays (fluorescent, colormetric)
Custom Competent Cell Preparation
(chemical, electrocompetent, 96 well
format)
(chemical, electrocompetent, 96 well
format)
Subcloning
Bacterial or Yeast Growth
Custom Protein Formulations
Hi-Pfi High-Fidelity DNA Polymerase
| Cat. number | Concentration (U/ul) | Size (ul) | Price (US Dollar) | |
| 6006 | 5 | 100 | $120.00 |  |
You can also place an order by faxing a completed order form to 858-558-3740 or calling us at 858-558-3702.
Description:
The Hi-Pfi DNA polymerase is a thermostable DNA polymerase derived from the hyperthermophile Pyrococcus furiosus. It's 3'-5' exonuclease (proofreading) activity makes it one of the most accurate DNA polymerases available.
Protein Uses:
High fidelity PCR
Site-directed mutagenesis procedure
Blunt-end and mega-primer PCR cloning
Library screening
Additional Information:
Recombinant protein expressed in E.coli
Thermostable – retains 95% of activity after 2 hours at 95°C
Eight times more accurate than Taq DNA polymerase
Capable of incorporating biotin, digoxigenin, and fluorescently–labled nucleotides
Use of primers and templates containing dUTP or dITP stalls a portion of the polymerase activity and may affect PCR results