T7 SSB
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase, Phosphatase (-)
T4 DNA Polymerase
MMLV reverse transcriptase
Vaccinia Topoisomerase I
Protein Expression Testing
AKTA FPLC (ion-exchange, IMAC)
Protein Purification Process Development
Non-Tagged Protein Purification
Complete Batch Records
Manufacturing Pilot Process
Scale-Up
Custom Protein Formulations
Tissue Culture (primary cells, adherent and suspension cell lines)
Viability Assays (fluorescent, colormetric)
Custom Competent Cell Preparation
(chemical, electrocompetent, 96 well
format)
(chemical, electrocompetent, 96 well
format)
Subcloning
Bacterial or Yeast Growth
Custom Protein Formulations
T4 DNA Polymerase
| Cat. number | Concentration (mg/ml) | Size (ul) | Size (units) | Price (US Dollar) | |
| 4004 | 0.5 | 100 | 750 | $120.00 |  |
| 4005 | 1.0 | 300 | 4,500 | $480.00 | |
You can also place an order by faxing a completed order form to 858-558-3740 or calling us at 858-558-3702.
Description:
T4 DNA Polymerase is the replicative polymerase from the E.coli phage T4. This polymerase catalyzes DNA synthesis in the 5' to 3' direction from primed templates. The enzyme features a robust 3'-5 exonuclease activity and has no 5'-3' exonuclease activity. T4 DNA polymerase creates blunt ends, by filling in the 5' end or removing 3' overhangs
Protein Uses:
Hi-fidelity synthesis
Site-directed mutagenesis
Probe labeling
Gap filling and second strand deletion sub-cloning
Additional Information:
This polymerase has low strand displacement capability
The 3'-5' exonuclease activity is extremely active and is enhanced by elevated temperatures, high enzyme concentrations or low dNTP concentrations.