
T7 SSB

T4 Polynucleotide Kinase

T4 Polynucleotide Kinase, Phosphatase (-)

T4 DNA Polymerase

MMLV reverse transcriptase

Vaccinia Topoisomerase I

Protein Expression Testing

AKTA FPLC (ion-exchange, IMAC)

Protein Purification Process Development

Non-Tagged Protein Purification

Complete Batch Records

Manufacturing Pilot Process

Scale-Up

Custom Protein Formulations

Tissue Culture (primary cells, adherent and suspension cell lines)

Viability Assays (fluorescent, colormetric)

Custom Competent Cell Preparation
(chemical, electrocompetent, 96 well
format)
(chemical, electrocompetent, 96 well
format)

Subcloning

Bacterial or Yeast Growth

Custom Protein Formulations
T4 Polynucleotide Kinase
You can also place an order by faxing a completed order form to 858-558-3740 or calling us at 858-558-3702.
Description:
T4 Polynucleotide Kinase is derived from the E.coli bacteriophage T4 and expressed in E.coli. T4 Polynucleotide Kinase catalyzes the exchange of PO4 from the γ - position of ATP to the 5' end of DNA and RNA as well as nucleoside 3' monophosphates. T4 PNK will also remove the 3' phosphoryl from dexoynucleoside 3' mono and di-phosphates as well as from 3' phosphoryl polynucleotides.
Protein Uses:

End-labeling of DNA and RNA for probes

Labeling of gaps in double-stranded substrates
Additional Information:

Nicks are not as efficiently phosphorylated