T7 SSB
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase, Phosphatase (-)
T4 DNA Polymerase
MMLV reverse transcriptase
Vaccinia Topoisomerase I
Protein Expression Testing
AKTA FPLC (ion-exchange, IMAC)
Protein Purification Process Development
Non-Tagged Protein Purification
Complete Batch Records
Manufacturing Pilot Process
Scale-Up
Custom Protein Formulations
Tissue Culture (primary cells, adherent and suspension cell lines)
Viability Assays (fluorescent, colormetric)
Custom Competent Cell Preparation
(chemical, electrocompetent, 96 well
format)
(chemical, electrocompetent, 96 well
format)
Subcloning
Bacterial or Yeast Growth
Custom Protein Formulations
T4 Polynucleotide Kinase, Phosphatase (-)
| Cat. number | Concentration (U/ul) | Size (ul) | Size (units) | Price (US Dollar) | |
| 9010 | 10 | 50 | 500 | $130.00 |  |
| 9011 | 100 | 50 | 5,000 | $800.00 | |
You can also place an order by faxing a completed order form to 858-558-3740 or calling us at 858-558-3702.
Description:
T4 Polynucleotide Kinase , phosphatase (-) is derived from the E.coli bacteriophage T4 and expressed in E.coli. T4 Polynucleotide Kinase catalyzes the exchange of PO4 from the the γ-position of ATP to the 5' end of DNA and RNA as well as nucleoside 3' monophosphates. This phosphatase (-) T4 Polynucleotide Kinase has the amino acid at position 167 changed from Aspartic Acid (D) to alanine (A). This mutation abolishes the 3' phosphatase activity while retaining full kinase activity.
Protein Uses:
End-labeling of DNA and RNA for probes
Labeling of gaps in double-stranded substrates
Additional Information:
Nicks are not as efficiently phosphorylated