
T7 SSB

T4 Polynucleotide Kinase

T4 Polynucleotide Kinase, Phosphatase (-)

T4 DNA Polymerase

MMLV reverse transcriptase

Vaccinia Topoisomerase I

Protein Expression Testing

AKTA FPLC (ion-exchange, IMAC)

Protein Purification Process Development

Non-Tagged Protein Purification

Complete Batch Records

Manufacturing Pilot Process

Scale-Up

Custom Protein Formulations

Tissue Culture (primary cells, adherent and suspension cell lines)

Viability Assays (fluorescent, colormetric)

Custom Competent Cell Preparation
(chemical, electrocompetent, 96 well
format)
(chemical, electrocompetent, 96 well
format)

Subcloning

Bacterial or Yeast Growth

Custom Protein Formulations
T7 DNA Single Stranded Binding (SSB) Protein
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Description:
T7 SSB (gene 2.5 protein, gp2.5) is functionally similar to Escherichia coli SSB and the gene 32 protein of bacteriophage T4, despite little sequence homology.
Protein Uses:

Stimulates DNA polymerase activity

Increases efficiency of RNA primer synthesis (through T7 DNA polymerase and primase-helicase interaction)
Additional Information:

More efficient at mediating homologous base-pairing than E.coli SSB protein

May assist in coordinating leading and lagging strand synthesis at the replication fork