T7 SSB
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase, Phosphatase (-)
T4 DNA Polymerase
MMLV reverse transcriptase
Vaccinia Topoisomerase I
Protein Expression Testing
AKTA FPLC (ion-exchange, IMAC)
Protein Purification Process Development
Non-Tagged Protein Purification
Complete Batch Records
Manufacturing Pilot Process
Scale-Up
Custom Protein Formulations
Tissue Culture (primary cells, adherent and suspension cell lines)
Viability Assays (fluorescent, colormetric)
Custom Competent Cell Preparation
(chemical, electrocompetent, 96 well
format)
(chemical, electrocompetent, 96 well
format)
Subcloning
Bacterial or Yeast Growth
Custom Protein Formulations
Vaccinia Topoisomerase I
| Cat. number | Concentration (U/ul) | Size (ul) | Size (units) | Price (US Dollar) | |
| 10004 | 10 | 100 | 1,000 | $120.00 |  |
| 10005 | 100 | 50 | 5,000 | $410.00 | |
You can also place an order by faxing a completed order form to 858-558-3740 or calling us at 858-558-3702.
Description:
Vaccinia DNA Topoisomerase I is derived from the Topoisomerase I gene from Orthopoxvirus Vaccinia and expressed in E.coli. Vaccinia topoisomerase is a type I eukaryotic topoisomerase that removes both positive (right) and negative (left) supercoils from covalently closed DNA. The enzyme reaction leaves a covalently closed, circular DNA product with reduced topology.
Protein Uses:
To examine the degree and extent of DNA supercoiling
To determine the effects of DNA topology on transcription and chromatin reconstitution
To expose restriction sites by reducing DNA supercoiling
Additional Information:
Activity is increased by low concentrations of Mg2+, but Mg2+ is not required for enzyme function.