T7 SSB
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase, Phosphatase (-)
T4 DNA Polymerase
MMLV reverse transcriptase
Vaccinia Topoisomerase I
Protein Expression Testing
AKTA FPLC (ion-exchange, IMAC)
Protein Purification Process Development
Non-Tagged Protein Purification
Complete Batch Records
Manufacturing Pilot Process
Scale-Up
Custom Protein Formulations
Tissue Culture (primary cells, adherent and suspension cell lines)
Viability Assays (fluorescent, colormetric)
Custom Competent Cell Preparation
(chemical, electrocompetent, 96 well
format)
(chemical, electrocompetent, 96 well
format)
Subcloning
Bacterial or Yeast Growth
Custom Protein Formulations
Exo (-) Klenow
| Cat. number | Concentration (U/ul) | Size (ul) | Size (units) | Price (US Dollar) | |
| 1001 | 5 | 100 | 500 | $65.00 |  |
| 1002 | 40 | 50 | 2,000 | $240.00 | |
| 1003 | 200 | 20 | 4,000 | $475.00 | |
You can also place an order by faxing a completed order form to 858-558-3740 or calling us at 858-558-3702.
Description:
This is an N-terminal truncation of the E. coli DNA Polymerase I protein, at amino acid #323. This truncation results in the deletion of the
5' to 3' exonuclease domain, but leaves the polymerase function unaffected. The introduction of two additional mutations (D355A and
E357A) results in the abolition of the 3' to 5' exonuclease activity.Available in three concentrations and supplied with 10X reaction buffer.
The highest-concentration (200 U/µl) enzyme is also supplied with 1X dilution buffer.
Protein Uses:
Didexoy sequncing.
Fill-in of restriction fragments to create blunt ends.
Second strand cDNA synthesis for labeling and for use in mutagenesis protocols.
Labeling of DNA with nucleotide analogs (e.g. for use as microarray probes).
Dye incorporation assays.
Probe labeling.
Additional Information:
Single recombinant protein, not a fusion construct, expressed in E. coli.
High purity, low DNA content.
Specific activity: 15,000 - 20,000 U/mg.
Has strand displacement capability.
No intrinsic exonuclease activity.
Error rate of 1/10000 bases.