
T7 SSB

T4 Polynucleotide Kinase

T4 Polynucleotide Kinase, Phosphatase (-)

T4 DNA Polymerase

MMLV reverse transcriptase

Vaccinia Topoisomerase I

Protein Expression Testing

AKTA FPLC (ion-exchange, IMAC)

Protein Purification Process Development

Non-Tagged Protein Purification

Complete Batch Records

Manufacturing Pilot Process

Scale-Up

Custom Protein Formulations

Tissue Culture (primary cells, adherent and suspension cell lines)

Viability Assays (fluorescent, colormetric)

Custom Competent Cell Preparation
(chemical, electrocompetent, 96 well
format)
(chemical, electrocompetent, 96 well
format)

Subcloning

Bacterial or Yeast Growth

Custom Protein Formulations
Taq DNA Polymerase
You can also place an order by faxing a completed order form to 858-558-3740 or calling us at 858-558-3702.
Description:
This is a wild-type recombinant DNA polymerase, derived from T. aquaticus, expressed in E. coli. The Taq polymerase is a thermo-stable DNA polymerase with 5' to 3' polymerase activity and low 5' to 3' exonuclease activity.
Available in two concentrations and supplied with 10X reaction buffer.
The higher-concentration (50 U/µl) enzyme is also supplied with 1X dilution buffer.
Protein Uses:

Primer extension.

Polymerase chain reaction.

DNA sequencing.

Site-directed mutagenesis.
Additional Information:

Single recombinant polypeptide of 94 kDa, expressed in E.coli.

High purity, low DNA content.

Specific activity: 100,000 - 150,000 U/mg.

Exhibits deoxynucleotidyl-transferase activity, which results in the addition of extra adenines at the 3' end of PCR products - suitable for use in TA cloning.

Half-life of 45 minutes @ 94°C.

Error rate of 1/45,000 bases.